Mouse Anti-Human CD45 Monoclonal IgG1

Mfr.No.	SMC-256, SMC-256D, SMC-256D-A390, SMC-256D-A488, SMC-256D-A594, SMC-256D-BI, SMC-256D-FITC
Immunogen Species	Human
Alternative Names	B220 Antibody, CD 45 Antibody, CD45R Antibody, GP180 Antibody, LCA Antibody, Protein tyrosine phosphatase receptor type c polypeptide Antibody, PTPRC_HUMAN Antibody, T200 glycoprotein Antibody
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	A 1:1000 dilution of SMC-256 was sufficient for detection of CD45 in 10 ug of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human CD45 Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Purified recombinant fragment of human CD45 expressed in E. Coli, Homo Sapiens (Human)
Immunogen Region	Purified recombinant fragment of human CD45 expressed in E. Coli
Isotype	IgG1
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	P08575
Accession Number	NP_563578.2
Gene Id	5788
Target	CD45
Clone No	12A9
Research Area	Immunology, Adaptive Immunity, B Cell CD Markers, T Cells, T Cell CD Markers, Cell Type Markers, CD Markers, Non-Lineage Markers, Cancer, Cancer Growth Inhibitors, Tyrosine Kinase Inhibitors, Cell Markers, Glial Markers, Microglia Markers, Stem Cells, Hematopoietic Progenitors, HP Surface Molecules, B Lymphocytic Lineage, T Lymphocytic Lineage, NK Cell Lineage, Myeloid, Dendritic Cell Lineage, Monocytic Lineage, Neutrophil Lineage, Myeloid Mast Cell Lineage, Regulatory T Cells, Angiogenesis, B Cells, Neuroscience, Lymphoid

Buffer	PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Scientific Background	CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, broadly expressed amount hematopoietic cells. There are kmultiple isoforms of CD45 distributed throughout the immune system, and they arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous CD45 proteins. (~147KD).
Storage	-20ºC
Storage Buffer	PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB
Description	WB ; IHC
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Barclay A., et al. (1997) The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge I.S., et al. (1993) Annu. Rev. Immunol. 12:85. 3. Kishihara K., et al. (1993) Cell. 74:143. 4. Pulido R., et al. (1988) J. Immunol. 140:3851.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902183-1	SMC-256D	Unconjugated	100 ul	$286	Add to Cart
BHA11902183-2	SMC-256D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11902183-3	SMC-256D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11902183-4	SMC-256D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11902183-5	SMC-256D-BI	Biotin	100 ul	$334	Add to Cart
BHA11902183-6	SMC-256D-FITC	FITC	100 ul	$330	Add to Cart

CD45 Antibody, Clone 12A9 - SMC-256