This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alteative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A recombinant human full-length CD44v3-10 protein was used as the immunogen for this CD44v4/5 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P16070
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD44v4/5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, WB, IHC-P
Description
Flow cytometry: 1-2ug/million cells in 0.1ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml