CD44 is also known as HCAM, LHR and MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
Amino acids RFAGVFHVEKNGRYSISRTEAADLCKAF from the human protein were used as the immunogen for the HCAM antibody.
Isotype
IgG2b
Predicted Reactivity
Human, Rat
Reactivity
Human, Rat
Recombinant
No
Uniprot
P16070
Clone No
7H7
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the HCAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IF, FACS
Dilution
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells