CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids E21-R193 from the human protein were used as the immunogen for the CD40 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Cytoplasmic, cell membrane
Uniprot
P25942
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CD40 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, ELISA
Dilution
Western blot: 0.5-1ug/ml,ELISA: 0.5-1ug/ml (human protein tested); request BSA-free format for coating