CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rat
Immunogen Region
Mouse CTL clone V4 was used as the immunogen for the CD4 antibody.
Isotype
Rat IgG2b, kappa
Species Reactivity
Mouse
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P06332
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
Neutralization, ELISA, FACS, IF
Description
ELISA (order BSA-free format for coating & Azide),Neutralization Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml