This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
A full length recombinant human protein was used as the immunogen for the CD34 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).