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Home / Primary Antibodies

CD34 Antibody (V3001SAF)

SKU : BHA17124599

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NSJ Bioreagents

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Mfr.No.	V3001SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI inteational workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant full-length human protein was used as the immunogen for the CD34 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the CD34 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P28906

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124599	V3001SAF-100UG	100 ug	$499		Add to Cart

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