CD34 is a monomeric cell surface antigen with a glycosylated molecular mass of approximately 110 KD. It is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene is located on human chromosome 1. It plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 151-385) was used as the immunogen for this CD34 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Cytoplasmic, membranous
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CD34 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IF
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml