Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Recombinant human protein was used as the immunogen for this CD28 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the CD28 antibody should be determined by the researcher.
Format
CF488 Conjugate
Purity
Protein G affinity chromatography
Storage
Store the CD28 antibody at 2-8oC, protected from light.
Applications
FACS, IF
Description
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100