Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NF B and SAPK/JNK and induces apoptosis.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 28-170 from the human protein was used as the immunogen for the CD27 antibody.
Isotype
Mouse IgG2c, kappa
Species Reactivity
Human
Note
Optimal dilution of the CD27 antibody should be determined by the researcher.
Uniprot
P26842
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT