CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human
Note
Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P11836
Format
Purified
Purity
Protein A affinity chromatography
Storage
Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Applications
FACS, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT