CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Steberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Purified human neutrophils were used as the immunogen for the CD15 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the CD15 antibody should be determined by the researcher.
Uniprot
P22083
Format
Purified
Purity
Protein A affinity
Storage
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF, IHC-P
Description
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 5-10ug/ml for 30 min at RT