Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A human recombinant partial protein (within amino acids 25-148) was used as immunogen for this CD14 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.
Uniprot
P08571
Format
Purified
Purity
Protein G affinity chromatography
Storage
CD14 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Applications
WB, FACS, IHC-P
Description
Weste blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT