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Home / Primary Antibodies / Polyclonal Antibodies

Calreticulin Antibody - SPC-122

BHA11906583
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+1 866.986.9598
Email
[email protected]
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Swissprot P27797
Accession Number NP_004334.1
Gene Id 811
Target Calreticulin
Research Area Organelle Proteins| Tags and Cell Markers, Cell Signaling, Organelle Markers, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~63kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF, FCM
Description WB ; IHC ; ICC ; IF ; IP ; FCM
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Johnson S., et al. (2001) Trends Cell Biol 11: 122-129.

2. Smith M.J., et al. (1989) EMBO J. 8: 3581-3586.

3. Ellgaard L., et al. (2001) Curr Opin Cell Biol. 13: 431-437.

4. Krause K.H., and Michalak M. (1997) Cell. 88: 439-443.

5. Nash P.D., et al. (1994) Mol Cell Biochem. 135: 71-78.

6. Heal R., and McGivan J. (1998) Biochem J. 329: 389-394.

7. Lucero H.A., et al. (1998) J Biol Chem. 273: 9857-9863.

8. Tanaka S., et al. (2000) J Biol Chem. 275: 10388-10393.

9. Yoon G.S., et al. (2000) Cancer Res. 60: 1117-1120.

10. Antoniou A.N., et al. (2002) Immunology 106: 182-189.

11. Wada I., et al. (1997) EMBO J. 16: 5420-5432.

12. Laguerre D.B., et al. (1998) J Vir. 72: 4940-4949.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906583-1 SPC-122B Unconjugated 200 ul $275
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BHA11906583-2 SPC-122A Unconjugated 50 ul $125
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BHA11906583-3 SPC-122B-A390 ATTO 390 200 ul $377
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BHA11906583-4 SPC-122B-A488 ATTO 488 200 ul $374
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BHA11906583-5 SPC-122B-A594 ATTO 594 200 ul $374
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BHA11906583-6 SPC-122B-ALP Alkaline Phosphatase 200 ul $361
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BHA11906583-7 SPC-122B-APC APC 200 ul $373
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BHA11906583-8 SPC-122B-BI Biotin 200 ul $363
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BHA11906583-9 SPC-122B-FITC FITC 200 ul $355
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BHA11906583-10 SPC-122B-HRP HRP 200 ul $349
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BHA11906583-11 SPC-122B-PCP PerCP 200 ul $373
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BHA11906583-12 SPC-122B-RPE RPE 200 ul $366
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BHA11906583-13 SPC-122S Unconjugated 12 ul $44
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