Calponin-1/CNN1, a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
A synthetic peptide from the C-terminus of the human protein was used as the immunogen for the recombinant CNN1 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the recombinant CNN1 antibody should be determined by the researcher.
Uniprot
P51911
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant CNN1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT