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Home / Primary Antibodies / Polyclonal Antibodies

Calnexin-NT Antibody - SPC-127

BHA11906642
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Email
[email protected]
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Swissprot P24643
Accession Number NP_001003232.1
Gene Id 403908
Target Calnexin
Research Area Cell Signaling, Tags and Cell Markers, Organelle Markers
Buffer Rabbit Antiserum
Dilutions WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Purification Rabbit Antiserum
Scientific Background Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects the N-terminal domain of Calnexin ~90kDa.
Storage -20ºC
Storage Buffer Rabbit Antiserum *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90.

2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229.

3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644.

4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819.

5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460.

6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103.

7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.

8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649.

9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906642-1 SPC-127B Unconjugated 200 ul $370
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BHA11906642-2 SPC-127A Unconjugated 50 ul $166
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BHA11906642-3 SPC-127B-A390 ATTO 390 200 ul $471
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BHA11906642-4 SPC-127B-A488 ATTO 488 200 ul $468
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BHA11906642-5 SPC-127B-A594 ATTO 594 200 ul $468
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BHA11906642-6 SPC-127B-ALP Alkaline Phosphatase 200 ul $455
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BHA11906642-7 SPC-127B-APC APC 200 ul $466
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BHA11906642-8 SPC-127B-BI Biotin 200 ul $458
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BHA11906642-9 SPC-127B-FITC FITC 200 ul $450
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BHA11906642-10 SPC-127B-HRP HRP 200 ul $443
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BHA11906642-11 SPC-127B-PCP PerCP 200 ul $466
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BHA11906642-12 SPC-127B-RPE RPE 200 ul $460
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BHA11906642-13 SPC-127S Unconjugated 12 ul $44
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