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Home / Antibodies

Caldesmon / CAD Antibody Cocktail (HMW) (V2943IHC)

SKU : BHA17124552

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NSJ Bioreagents

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Mfr.No.	V2943IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant full-length human protein (CALD1/820) and crude human uterus extract (h-CALD) were used as the immunogen for the CAD antibody cocktail.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the CAD antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q05682

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CAD antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124552	V2943IHC-7ML	7 ml	$499		Add to Cart

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