Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that CDH16 (kidney-specific cadherin, Cadherin 16) is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, CDH16 is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. CDH16 has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human, Mouse, Rat
Note
Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
O75309
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant Cadherin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, FACS, IHC-P
Description
Weste blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT