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BUB1 Antibody

SKU : BHA10558867

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA203302
Alternative Names	BUB1A; BUB1L; hBUB1; MBUB1; Mitotic checkpoint BUB1
Categories	Primary Antibodies
Clonality	polyclonal
Description	Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.Lengauer C., Genomics 58:181-187(1999).Taylor S.S., J. Cell Sci. 117:1577-1589(2004).Yu H., Mol. Biol. Cell 17:3705-3716(2006).
Host	Rabbit
Immunogen	Synthesized peptide derived from internal of Human BUB1.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. Lengauer C., Genomics 58:181-187(1999). Taylor S.S., J. Cell Sci. 117:1577-1589(2004). Yu H., Mol. Biol. Cell 17:3705-3716(2006).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of total BUB1 protein.
Subcellular Location	Nucleus, Chromosome, centromere, kinetochore

Uniprot	O43683
Database Links	HGNC:1148 OMIM:602452 KEGG:hsa:699 STRING:9606.ENSP00000302530 UniGene:Hs.469649
Function	Serine/threonine-protein kinase that performs 2 crucial functions during mitosis
Pathway	Cell cycle MAPK Erk pathway
Protein Families	Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
Tissue Specificity	High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:3000

Tested Application

ELISA,WB;WB:1:500-1:3000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558867	CSB-PA203302	100ul	$297		Add to Cart

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