It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Bromodeoxyuridine was used as the immunogen for this anti-Bromodeoxyuridine antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
All species
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
Not Applicable
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the anti-Bromodeoxyuridine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT