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Home / Primary Antibodies

Bromodeoxyuridine / BrdU Antibody (V3108SAF)

SKU : BHA17124749

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NSJ Bioreagents

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Mfr.No.	V3108SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	BrdU conjugated to hemocyanine was used as the immunogen for the Bromodeoxyuridine antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	All species
Note	Optimal dilution of the Bromodeoxyuridine antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Not Applicable

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Bromodeoxyuridine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	FACS, IF, IHC-P
Description	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124749	V3108SAF-100UG	100 ug	$499		Add to Cart

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