It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Isotype
Mouse IgG1, kappa
Species Reactivity
All species
Note
Optimal dilution of the Bromodeoxyuridine antibody should be determined by the researcher.
Format
CF488 Conjugate
Purity
Protein G affinity chromatography
Storage
Store the Bromodeoxyuridine antibody at 2-8oC, protected from light.
Applications
FACS, IF
Description
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100