It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
Isotype
Rabbit IgG
Species Reactivity
All species
Note
Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
Uniprot
Not Applicable
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT