This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3(Fucalpha1-3/4)GlcNAcbeta-). Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Lewis y antibody should be determined by the researcher.
Uniprot
Not Applicable
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Lewis y antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF, IHC-P
Description
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT