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Home / Antibodies

Biotin / Vitamin B7 / Vitamin H Antibody (V9363)

SKU : BHA17119749

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NSJ Bioreagents

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Mfr.No.	V9363, V9363-20UG, V9363-100UG
Clonality	Monoclonal (mouse origin)
Description	It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	All species
Note	Optimal dilution of the Biotin antibody should be determined by the researcher.

Uniprot	Not Applicable

Format	Purified
Purity	Protein A/G affinity
Storage	Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Applications	WB, FACS, IF, IHC-P
Description	Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

Product Manual	Datasheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17119749-1	V9363-20UG	20ug	$219		Add to Cart
BHA17119749-2	V9363-100UG	100ug	$499		Add to Cart

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