Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Full length recombinant human protein was used as the immunogen for the B2M antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Titering of the B2M antibody may be required for optimal performance.>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P61769
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the B2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IF, IHC-P, FACS
Description
Weste blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)