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Home / Primary Antibodies

Basic Cytokeratin Antibody (V3116SAF)

SKU : BHA17124765

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NSJ Bioreagents

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Mfr.No.	V3116SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q01546

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124765	V3116SAF-100UG	100 ug	$499		Add to Cart

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