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Home / Primary Antibodies

Basic Cytokeratin Antibody (HMW / Type II) (V7399IHC)

SKU : BHA17126428

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NSJ Bioreagents

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Mfr.No.	V7399IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. This MAb recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Solubilized cytokeratin extract from human stratum coeum was used as the immunogen.
Isotype	Mouse IgG1
Species Reactivity	Human
Note	Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q01546

Format	Purified
Purity	Protein G purified
Storage	Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/basic-cytokeratin-antibody-spm116-v7399" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126428	V7399IHC-7ML	7 ml	$499		Add to Cart

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