Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin, which can bind to beta-catenin and can also bind actin, and beta-catenin. b-Catenin binds the cytoplasmic domain of some cadherins as an adherens junction protein. In normal tissues, b-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A synthetic peptide corresponding to the N-terminus of the human protein (MATQADLMELDMAMEPDRK) was used as the immunogen for this b-Catenin antibody (100% homologous in human, mouse and rat).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
P35222
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the b-Catenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IHC-F, ICC
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml