Rabbit Anti-Human Aurora B (pSer227) Polyclonal

Mfr.No.	SPC-920, SPC-920D, SPC-920D-A390, SPC-920D-A488, SPC-920D-A594, SPC-920D-BI, SPC-920D-FITC
Immunogen Species	Human
Alternative Names	AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody
Antigen	Aurora B
Categories	Antibodies
Cellular Localization	Cytoskeleton, Nucleus, Cytoplasm
Certificate Of Analysis	A 1:250 dilution of SPC-920 was sufficient for detection of Aurora B (pSer227) in 10 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human Aurora B (pSer227) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A phospho-specific peptide corresponding to residues surrounding Ser227 of human Aurora B (AA222-228), Homo Sapiens (Human)
Immunogen Region	A phospho-specific peptide corresponding to residues surrounding Ser227 of human Aurora B (AA222-228)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase

Swissprot	Q96GD4
Accession Number	NP_004208
Gene Id	9212
Target	Aurora B (pSer227)
Research Area	Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, Aurora, Epigenetics and Nuclear Signaling, Chromatin Modifying Enzymes, Cancer, Cell Cycle

Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions	WB (1:250); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase
Scientific Background	Aurora B is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects 39.28 kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	AM, WB
Description	WB ; AM
Dilution	WB (1:250); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11911376-1	SPC-920D	Unconjugated	100 ul	$331	Add to Cart
BHA11911376-2	SPC-920D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11911376-3	SPC-920D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11911376-4	SPC-920D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11911376-5	SPC-920D-BI	Biotin	100 ul	$380	Add to Cart
BHA11911376-6	SPC-920D-FITC	FITC	100 ul	$375	Add to Cart

Aurora B Antibody (pSer227) - SPC-920