The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of of amino acids 1-119 from the human protein was used as the immunogen for the ATG5 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the ATG5 antibody should be determined by the researcher.
Uniprot
Q9H1Y0
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the ATG5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, ELISA, IHC-P
Description
ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format),Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT