Rabbit Anti-Human ATG5 Polyclonal

Mfr.No.	SPC-611, SPC-611D, SPC-611D-A390, SPC-611D-A488, SPC-611D-A594, SPC-611D-ALP, SPC-611D-APC, SPC-611D-BI, SPC-611D-FITC, SPC-611D-HRP, SPC-611D-PCP, SPC-611D-RPE, SPC-611S
Immunogen Species	Human
Alternative Names	APG5-like Antibody, ASP Antibody, hAPG5 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:1000 dilution of SPC-611 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human ATG5 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the mid-protein of human ATG5, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the mid-protein of human ATG5
Launch Date	2015/10/01
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	Q9H1Y0
Accession Number	NP_001273035.1
Gene Id	9474
Target	ATG5
Research Area	Cancer, Apoptosis, Autophagy, Cardiovascular System, Heart, Cell Signaling, Metabolism, Metabolism processes

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11908204-1	SPC-611D	Unconjugated	100 ug	$331	Add to Cart
BHA11908204-2	SPC-611D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11908204-3	SPC-611D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11908204-4	SPC-611D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11908204-5	SPC-611D-ALP	Alkaline Phosphatase	100 ug	$377	Add to Cart
BHA11908204-6	SPC-611D-APC	APC	100 ug	$383	Add to Cart
BHA11908204-7	SPC-611D-BI	Biotin	100 ug	$380	Add to Cart
BHA11908204-8	SPC-611D-FITC	FITC	100 ug	$375	Add to Cart
BHA11908204-9	SPC-611D-HRP	HRP	100 ug	$371	Add to Cart
BHA11908204-10	SPC-611D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11908204-11	SPC-611D-RPE	RPE	100 ug	$381	Add to Cart
BHA11908204-12	SPC-611S	Unconjugated	12 ug	$44	Add to Cart

ATG5 Antibody - SPC-611