Rabbit Anti-Human ATG4D Polyclonal

Mfr.No.	SPC-635, SPC-635D, SPC-635D-A390, SPC-635D-A488, SPC-635D-A594, SPC-635D-ALP, SPC-635D-APC, SPC-635D-BI, SPC-635D-FITC, SPC-635D-HRP, SPC-635D-PCP, SPC-635D-RPE, SPC-635S
Immunogen Species	Human
Alternative Names	Autophagin-4 Antibody, APG4D Antibody, ATG4D Antibody, Cysteine protease ATG4D Antibody, AUTL4 Antibody, Autophagy-related cysteine endopeptidase 4 Antibody, EC:3.4.22.- Antibody, AUT-like 4 cysteine endopeptidase Antibody, Autophagy-related protein 4 homolog D Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:1000 dilution of SPC-635 was sufficient for detection of ATG4D in 15 ug of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human ATG4D Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108), Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human
Tissue Specificity	Mainly expressed in skeletal muscle and, to a lower extent, in testis.

Swissprot	Q86TL0
Accession Number	NP_001268433.1
Gene Id	84971
Target	ATG4D
Research Area	Cardiovascular System, Heart, Cancer, Autophagy

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Mainly expressed in skeletal muscle and, to a lower extent, in testis.
Scientific Background	ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~53 kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. "Entrez Gene: Autophagy related 4D, cysteine peptidase". 2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11908508-1	SPC-635D	Unconjugated	100 ug	$331	Add to Cart
BHA11908508-2	SPC-635D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11908508-3	SPC-635D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11908508-4	SPC-635D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11908508-5	SPC-635D-ALP	Alkaline Phosphatase	100 ug	$377	Add to Cart
BHA11908508-6	SPC-635D-APC	APC	100 ug	$383	Add to Cart
BHA11908508-7	SPC-635D-BI	Biotin	100 ug	$380	Add to Cart
BHA11908508-8	SPC-635D-FITC	FITC	100 ug	$375	Add to Cart
BHA11908508-9	SPC-635D-HRP	HRP	100 ug	$371	Add to Cart
BHA11908508-10	SPC-635D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11908508-11	SPC-635D-RPE	RPE	100 ug	$381	Add to Cart
BHA11908508-12	SPC-635S	Unconjugated	12 ug	$44	Add to Cart

ATG4D Antibody - SPC-635