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Home / Antibodies

ATG4B Antibody (F54715)

SKU : BHA17103179

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NSJ Bioreagents

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Mfr.No.	F54715, F54715-0.08ML, F54715-0.4ML
Clonality	Polyclonal
Description	Macroautophagy is the major inducible pathway for the general tuover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Formulation	In 1X PBS, pH 7.4, with 0.09% sodium azide
Host	Rabbit
Immunogen Region	A portion of amino acids 358-390 from the human protein was used as the immunogen for the ATG4B antibody.
Isotype	Ig
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cytoplasmic

Uniprot	Q9Y4P1

Format	Purified
Purification	Purified
Storage	Aliquot the ATG4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Applications	IHC-P, WB, IF
Dilution	Immunohistochemistry (FFPE): 1:25, Weste blot: 1:500-1:2000, Immunofluorescence: 1:25

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17103179-1	F54715-0.08ML	0.08ml	$165		Add to Cart
BHA17103179-2	F54715-0.4ML	0.4ml	$379		Add to Cart

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