Rabbit Anti-Human ATG12 Polyclonal

Mfr.No.	SPC-607, SPC-607D, SPC-607D-A390, SPC-607D-A488, SPC-607D-A594, SPC-607D-ALP, SPC-607D-APC, SPC-607D-BI, SPC-607D-FITC, SPC-607D-HRP, SPC-607D-PCP, SPC-607D-RPE, SPC-607S
Immunogen Species	Human
Alternative Names	APG12-like Antibody, APG12 Antibody, ATG12_Human Antibody, HAPG12 Antibody, FBR93 Antibody
Antigen	ATG12
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:1000 dilution of SPC-607 was sufficient for detection of ATG12 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Conjugate	APC, Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, Biotin, FITC, HRP, PerCP, RPE
Description	Rabbit Anti-Human ATG12 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the N-terminal of human ATG12, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the N-terminal of human ATG12
Launch Date	2015/10/01
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	O94817
Accession Number	NP_001264712.1
Gene Id	9140
Target	ATG12
Research Area	Cancer, Autophagy, Cell Signaling, Epigenetics and Nuclear Signaling, Cardiovascular System, Heart

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	ATG12 (autophagy-related protein 12), also known as APG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the ATG12 family of proteins. ATG12 is a homolog of the yeast protein APG12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of APG12 covalently bound at the carboxy terminal glycine to lysine 149 of APG5. Similarly in humans, ATG12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, ATG10 and ATP (1-3). The ATG12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two ATG12 isoforms exist.
Shipping Temperature	Blue Ice or 4°C
Specificity	Predicted molecular weight at ~15.1kDa. Observed molecular weights at ~48-55kDa based on ATG12-ATG5 heterodimer.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Mizushima N., Sugita H., Yoshimori T., Ohsumi Y. (1999) J Biol Chem. 273(51): 33889-33892. 2. Mizushuma N., et al. (1998) Nature. 395: 395-398. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-5981.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11908128-1	SPC-607D	Unconjugated	100 ug	$331	Add to Cart
BHA11908128-2	SPC-607D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11908128-3	SPC-607D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11908128-4	SPC-607D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11908128-5	SPC-607D-ALP	Alkaline Phosphatase	100 ug	$377	Add to Cart
BHA11908128-6	SPC-607D-APC	APC	100 ug	$383	Add to Cart
BHA11908128-7	SPC-607D-BI	Biotin	100 ug	$380	Add to Cart
BHA11908128-8	SPC-607D-FITC	FITC	100 ug	$375	Add to Cart
BHA11908128-9	SPC-607D-HRP	HRP	100 ug	$371	Add to Cart
BHA11908128-10	SPC-607D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11908128-11	SPC-607D-RPE	RPE	100 ug	$381	Add to Cart
BHA11908128-12	SPC-607S	Unconjugated	12 ug	$44	Add to Cart

ATG12 Antibody - SPC-607