ERBB1 ; HER1 ; mENA ; PIG61 ; Proto-oncogene c-ErbB-1 ; Receptor Tyrosine Protein Kinase ; ErbB1 ; Urogastrone
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
7p11.2
Clonality
monoclonal
Description
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Host
Mouse
Immunogen
A synthetic peptide corresponding to aa 985-996 of human EGFR
Isotype
IgG1
Positive Control
A431 cells. Placenta, Breast, Colon or Bladder Carcinoma
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P00533
Gene Id
1956
Clone No
F4
Symbol
EGFR
Weight
~170kDa (wild type) and ~145kDa (vIII variant)
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
WB
Description
Optimal dilution for a specific application should be determined., WB
Dilution
Western Blotting
1-2μg/ml
Reviews of Anti-undefined Antibody F4 Unconjugated BHA10208200