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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Podocin NPHS2 Antibody Picoband®

SKU : BHA21006717

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Mfr.No.	PA1322, PA1322-10ug, PA1322-Biotin, PA1322-Cy3, PA1322-Dylight488, PA1322-Dylight550, PA1322-Dylight594, PA1322-FITC, PA1322-HRP, PA1322-APC, PA1322-PE, PA1322-iFluor647, PA1322-carrier-free
Alternative Names	Podocin
Background	Podocin (PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al. (2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence in the middle region of human NPHS2, different from the mouse sequence by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	37 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Podocin;NPHS2;
Tissue Specificity	Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	Q9NP85
Gene Name	NPHS2
Sequence	Belongs to the band 7/mec-2 family.
Subcellular Location	Isoform 1: Cell membrane ; Peripheral membrane protein .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Rat, Human, Mouse<br>

Reference	1. Boute, N.; Gribouval, O.; Roselli, S.; Benessy, F.; Lee, H.; Fuchshuber, A.; Dahan, K.; Gubler, M.-C.; Niaudet, P.; Antignac, C. : NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. Nature Genet. 24: 349-354, 2000. Note: Erratum: Nature Genet. 25: 125 only, 2000.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006717-1	PA1322	-	100 ug	$370		Add to Cart
BHA21006717-2	PA1322-10ug	Sample	10 ug	$99		Add to Cart
BHA21006717-3	PA1322-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006717-4	PA1322-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006717-5	PA1322-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006717-6	PA1322-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006717-7	PA1322-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006717-8	PA1322-FITC	FITC	100 ug	$570		Add to Cart
BHA21006717-9	PA1322-HRP	HRP	100 ug	$570		Add to Cart
BHA21006717-10	PA1322-APC	APC	100 ug	$670		Add to Cart
BHA21006717-11	PA1322-PE	PE	100 ug	$670		Add to Cart
BHA21006717-12	PA1322-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006717-13	PA1322-carrier-free	carrier-free	100 ug	$370		Add to Cart

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