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Home / Primary Antibodies / Polyclonal Antibodies

Anti-NDUFA1 Antibody Picoband®

SKU : BHA21006964

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Mfr.No.	PA1661, PA1661-10ug, PA1661-Biotin, PA1661-Cy3, PA1661-Dylight488, PA1661-Dylight550, PA1661-Dylight594, PA1661-FITC, PA1661-HRP, PA1661-APC, PA1661-PE, PA1661-iFluor647, PA1661-carrier-free
Alternative Names	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 1
Background	NDUFA1 (NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-NDUFA1 Antibody catalog # PA1661. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1, different from the related rat and mouse sequences by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	8 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 1;Complex I-MWFE;CI-MWFE;NADH-ubiquinone oxidoreductase MWFE subunit;NDUFA1;
Tissue Specificity	Primarily expressed in heart and skeletal muscle.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	O15239
Gene Name	NDUFA1
Sequence	Contains 1 death domain.
Subcellular Location	Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IF, IHC, IHC-F, ICC, WB, Flow Cytometry
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Rat, Human, Mouse<br> Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human<br> Flow Cytometry(Fixed), 1-3 ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Smeitink J, van den Heuvel L (1999). "Human mitochondrial complex I in health and disease.". Am. J. Hum. Genet. 64 (6): 150510.<br /><br />2. Au, H. C., Seo, B. B., Matsuno-Yagi, A., Yagi, T., Scheffler, I. E. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria. Proc. Nat. Acad. Sci. 96: 4354-4359, 1999.<br /><br />3. Feandez-Moreira, D., Ugalde, C., Smeets, R., Rodenburg, R. J. T., Lopez-Laso, E., Ruiz-Falco, M. L., Briones, P., Martin, M. A., Smeitink, J. A. M., Arenas, J. X-linked NDUFA1 gene mutations associated with mitochondrial encephalomyopathy. Ann. Neurol. 61: 73-83, 2007.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006964-1	PA1661	-	100 ug	$370		Add to Cart
BHA21006964-2	PA1661-10ug	Sample	10 ug	$99		Add to Cart
BHA21006964-3	PA1661-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006964-4	PA1661-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006964-5	PA1661-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006964-6	PA1661-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006964-7	PA1661-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006964-8	PA1661-FITC	FITC	100 ug	$570		Add to Cart
BHA21006964-9	PA1661-HRP	HRP	100 ug	$570		Add to Cart
BHA21006964-10	PA1661-APC	APC	100 ug	$670		Add to Cart
BHA21006964-11	PA1661-PE	PE	100 ug	$670		Add to Cart
BHA21006964-12	PA1661-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006964-13	PA1661-carrier-free	carrier-free	100 ug	$370		Add to Cart

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