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Home / Primary Antibodies / Polyclonal Antibodies

Anti-MCM2 Antibody Picoband®

SKU : BHA21006999

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Mfr.No.	PA1711, PA1711-10ug, PA1711-Biotin, PA1711-Cy3, PA1711-Dylight488, PA1711-Dylight550, PA1711-Dylight594, PA1711-FITC, PA1711-HRP, PA1711-APC, PA1711-PE, PA1711-iFluor647, PA1711-carrier-free
Alternative Names	DNA replication licensing factor MCM2
Background	MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-MCM2 Antibody catalog # PA1711. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed throu
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2, different from the related rat sequence by one amino acid.
Isotype	Rabbit IgG
Molecular Weight	102 kDa
Reactivity	Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;Mcm2;Bm28, Cdcl1, Kiaa0030, Mcmd2;
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P97310
Gene Name	MCM2
Sequence	Belongs to the MCM family.
Subcellular Location	Nucleus .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, By Heat<br> Immunocytochemistry , 0.5-1ug/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Mouse<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Mouse<br>

Reference	Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988.<br /><br />2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995.<br /><br />3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res Commun 177 (1): 395400.<br /><br /><br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006999-1	PA1711	-	100 ug	$370		Add to Cart
BHA21006999-2	PA1711-10ug	Sample	10 ug	$99		Add to Cart
BHA21006999-3	PA1711-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006999-4	PA1711-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006999-5	PA1711-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006999-6	PA1711-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006999-7	PA1711-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006999-8	PA1711-FITC	FITC	100 ug	$570		Add to Cart
BHA21006999-9	PA1711-HRP	HRP	100 ug	$570		Add to Cart
BHA21006999-10	PA1711-APC	APC	100 ug	$670		Add to Cart
BHA21006999-11	PA1711-PE	PE	100 ug	$670		Add to Cart
BHA21006999-12	PA1711-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006999-13	PA1711-carrier-free	carrier-free	100 ug	$370		Add to Cart

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