The antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
Isotype
IgG
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
100µg/100ul
Description
Specificity: HA-Tag polyclonal antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
Format
Liquid
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
WB: 1:500~1:1000, WB
Dilution
WB
1:500-1:1000~
Reference
Nam, G. et al. "Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces.." J Immunol 191(11):5730-42 (2013). DOI: 10.4049/jimmunol.1203062. PubMed: 24133166 . Application: Immuno Blotting.
Schvartz, T et al. "Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging." Mol. Biol. Cell 21:2747-2756 (2017). DOI: 10.1091/mbc.E17-03-0161. PubMed: 28835375. Application: Western Blot.
Reviews of Anti-HA Tag Antibody (Rabbit Polyclonal)