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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GRX2/GLRX2 Antibody Picoband®

SKU : BHA21002967

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Mfr.No.	A05331, A05331-10ug, A05331-Biotin, A05331-Cy3, A05331-Dylight488, A05331-Dylight550, A05331-Dylight594, A05331-FITC, A05331-HRP, A05331-APC, A05331-PE, A05331-iFluor647, A05331-carrier-free
Alternative Names	cytoskeleton associated protein 5
Background	GLRX2 (Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins (e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al. (2001) and Gladyshev et al. (2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al. (2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon (exon 1B) located upstream from the first exon used by the GLRX2A transcript (exon 1A), suggesting that alteative splicing generates the isoforms. Lundberg et al. (2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-GRX2/GLRX2 Antibody Picoband® catalog # A05331. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. I
Host	Rabbit
Immunogen Region	E.coli-derived human GRX2/GLRX2 recombinant protein (Position: M1-Q164).
Isotype	Rabbit IgG
Molecular Weight	18 kDa
Reactivity	Human, Mouse
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097
Tissue Specificity	Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain; highly expressed in the Purkinje cell bodies of the cerebellum.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	Q9NS18
Gene Id	51022
Gene Name	GLRX2
Subcellular Location	Centrosome. Spindle pole. Spindle. Kinetochore.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Gladyshev, V. N., Liu, A., Novoselov, S. V., Krysan, K., Sun, Q.-A., Kryukov, V. M., Kryukov, G. V., Lou, M. F.Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2.J. Biol. Chem. 276: 30374-30380, 2001.<br /><br />2. Lilig, C. H., Bedt, C., Vergnolle, O., Lonn, M. E., Hudemann, C., Bill, E., Holmgren, A.Characterization of human glutaredoxin 2 as iron-sulfur protein: a possible role as redox sensor.Proc. Nat. Acad. Sci. 102: 8168-8173, 2005.<br /><br />3. Lundberg, M., Johansson, C., Chandra, J., Enoksson, M., Jacobsson, G., Ljung, J., Johansson, M., Holmgren, A.Cloning and expression of a novel human glutaredoxin (Grx2) with mitochondrial and nuclear isoforms.J. Biol. Chem. 276: 26269-26275, 2001.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002967-1	A05331	-	100 ug	$370		Add to Cart
BHA21002967-2	A05331-10ug	Sample	10 ug	$99		Add to Cart
BHA21002967-3	A05331-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002967-4	A05331-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002967-5	A05331-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002967-6	A05331-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002967-7	A05331-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002967-8	A05331-FITC	FITC	100 ug	$570		Add to Cart
BHA21002967-9	A05331-HRP	HRP	100 ug	$570		Add to Cart
BHA21002967-10	A05331-APC	APC	100 ug	$670		Add to Cart
BHA21002967-11	A05331-PE	PE	100 ug	$670		Add to Cart
BHA21002967-12	A05331-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002967-13	A05331-carrier-free	carrier-free	100 ug	$370		Add to Cart

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