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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Glucose 6 phosphate isomerase/GPI Antibody Picoband®

SKU : BHA21000974

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Mfr.No.	PB10069, PB10069-10ug, PB10069-Biotin, PB10069-Cy3, PB10069-Dylight488, PB10069-Dylight550, PB10069-Dylight594, PB10069-FITC, PB10069-HRP, PB10069-APC, PB10069-PE, PB10069-iFluor647, PB10069-carrier-free
Alternative Names	Glucose-6-phosphate isomerase
Background	Glucose-6-phosphate isomerase (GPI), alteatively known as phosphoglucose isomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme that in humans is encoded by the GPI gene on chromosome 19. This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alteative splicing results in multiple transcript variants.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Glucose 6 phosphate isomerase/GPI Antibody Picoband® catalog # PB10069. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of mouse GPI, different from the related human sequence by sixteen amino acids, and from the related rat sequence by two amino acids.
Isotype	Rabbit IgG
Molecular Weight	64 kDa
Reactivity	Mouse
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Glucose-6-phosphate isomerase;GPI;5.3.1.9;Autocrine motility factor;AMF;Neuroleukin;NLK;Phosphoglucose isomerase;PGI;Phosphohexose isomerase;PHI;Gpi;Gpi1;
Tissue Specificity	Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P06745
Gene Id	14751
Gene Name	Gpi
Subcellular Location	Cytoplasm. Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.1-0.5ug/ml, Mouse

Reference	1. Haga A, Niinaka Y, Raz A (2000). "Phosphohexose isomerase/autocrine motility factor/neuroleukin/maturation factor is a multifunctional phosphoprotein.". Biochim. Biophys. Acta 1480(1-2): 23544. 2. Kugler W, Lakomek M (March 2000). "Glucose-6-phosphate isomerase deficiency". Baillieres Best Pract. Res. Clin. Haematol. 13 (1): 89101.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000974-1	PB10069	100 ug	-	$370		Add to Cart
BHA21000974-2	PB10069-10ug	10 ug	Sample	$99		Add to Cart
BHA21000974-3	PB10069-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000974-4	PB10069-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000974-5	PB10069-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000974-6	PB10069-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000974-7	PB10069-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000974-8	PB10069-FITC	100 ug	FITC	$570		Add to Cart
BHA21000974-9	PB10069-HRP	100 ug	HRP	$570		Add to Cart
BHA21000974-10	PB10069-APC	100 ug	APC	$670		Add to Cart
BHA21000974-11	PB10069-PE	100 ug	PE	$670		Add to Cart
BHA21000974-12	PB10069-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000974-13	PB10069-carrier-free	100 ug	carrier-free	$370		Add to Cart

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