Recombinant anti-GFRAL (GDNF Family Receptor alpha Like) monoclonal antibody which recognizes GFRAL. This recombinant antibody has been tested for specific binding to purified recombinant human GFRAL (BPS Bioscience #101013) in an ELISA binding assay.
assay conditions: An ELISA binding assay was performed with this antibody and its target protein, GFRAL (BPS Bioscience #101013). The antibody was coated onto an assay plate overnight at 2 ug/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of the target protein were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 2.4 nM
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Instructions for use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles
Molecular Weight
High Chain: 50 kDa; Low Chain: 24 kDa
Synonyms
DNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356/PRO34128