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Home / Primary Antibodies / Monoclonal Antibodies

Anti-FcGR3A-Anti-EGFR Bispecific Antibody

SKU : BHA18200026

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BPS Bioscience

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Mfr.No.	101535
Clonality	Comprised of two monoclonal antibodies.
Description	Anti-FcGR3A-anti-EGFR bispecific IgG antibody, which recognizes FcGR3A (also known as CD16A) and EGFR antigen binding proteins. The amino acid sequences correspond to the sequences from the bispecific EGFR/FcGR3 antigen-binding protein patent WO2019175368A1. This bispecific antibody has been tested for specific binding affinity to purified human FcGR3A and EGFR recombinant protein. assay conditions: Functional validation: The antibody was validated by measuring anti-FcGR3A-anti-EGFR binding to FcGR3A and EGFR antigens in ELISA assay. The FcGR3A protein (BPS Bioscience #79013) and EGFR protein were coated onto a 96-well plate oveight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified anti-FcGR3A-anti-EGFR antibody (BPS Bioscience #101535), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host	HEK293
Instructions for use	Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Molecular Weight	Heavy Chain: 76 kDa; Light Chain: 23 kDa + glycans
Species	Human
Synonyms	CD16-II, CD16a antigen, FcR-10, IgG Fc receptor III-2, FCG3, IGFR3, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1
Note	Avoid freeze/thaw cycles.

Uniprot	FcGR3A: P08637; EGFR: P00533

Format	Aqueous buffer solution.
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200026	101535	50 ug	$368.50		Add to Cart

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