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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Cdc25B Antibody Picoband®

SKU : BHA21000384

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Mfr.No.	PB9488, PB9488-10ug, PB9488-Biotin, PB9488-Cy3, PB9488-Dylight488, PB9488-Dylight550, PB9488-Dylight594, PB9488-FITC, PB9488-HRP, PB9488-APC, PB9488-PE, PB9488-iFluor647, PB9488-carrier-free
Alternative Names	M-phase inducer phosphatase 2
Background	Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Cdc25B Antibody Picoband® catalog # PB9488. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. ly dephosphorylates CDK1 and stimulates
Host	Rabbit
Immunogen Region	E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	M-phase inducer phosphatase 2;3.1.3.48;Dual specificity phosphatase Cdc25B;CDC25B;CDC25HU2;
Tissue Specificity	Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	P30305
Gene Id	994
Gene Name	CDC25B
Sequence	Belongs to the MPI phosphatase family.
Subcellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry(Fixed), 1-3 ug/1x10⁶ cells, Human

Reference	1. Bulavin, D. V., Higashimoto, Y., Popoff, I. J., Gaarde, W. A., Basrur, V., Potapova, O., Appella, E., Foace, A. J., Jr.Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase.Nature 411: 102-107, 2001. 2. Lane, S. A., Baker, E., Sutherland, G. R., Tonks, I., Hayward, N., Ellem, K.The human cell cycle gene CDC25B is located at 20p13.Genomics 15: 693-694, 1993.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000384-1	PB9488	100 ug	-	$370		Add to Cart
BHA21000384-2	PB9488-10ug	10 ug	Sample	$99		Add to Cart
BHA21000384-3	PB9488-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000384-4	PB9488-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000384-5	PB9488-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000384-6	PB9488-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000384-7	PB9488-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000384-8	PB9488-FITC	100 ug	FITC	$570		Add to Cart
BHA21000384-9	PB9488-HRP	100 ug	HRP	$570		Add to Cart
BHA21000384-10	PB9488-APC	100 ug	APC	$670		Add to Cart
BHA21000384-11	PB9488-PE	100 ug	PE	$670		Add to Cart
BHA21000384-12	PB9488-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000384-13	PB9488-carrier-free	100 ug	carrier-free	$370		Add to Cart

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