The T cell-specific receptor CD28 is an important regulator of the immune system. The ligation of CD28 and its ligands CD80/CD86 on the APC provides a critical costimulatory signal alongside T cell receptor (TCR) for T cell activation. The costimulatory signal can also be delivered by the interaction between CD28 and its agonist antibody.
Clonality
Monoclonal
Description
Recombinant monoclonal antibody recognizing human CD28. This antibody binds to CD28 on T cells and promotes T cell response. Not tested in other species. This antibody has been humanized to reduce immunogenicity and improve the therapeutic half-life.
assay conditions: Serial dilutions of anti-CD3 (BPS Bioscience, #71274) and CD28 antibodies were added to the IL-2 reporter-Jurkat cells, then incubated at 37°C incubator for 16 hrs. After the treatment, perform Luciferase assay using One-Step Luciferase assay system (BPS Bioscience, #60690)
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol
Molecular Weight
Heavy Chain: 51 kDa; Light Chain: 27 kDa
Species
Human
Synonyms
T-Cell-Specific Surface Glycoprotein, TP44
Note
Avoid freeze/thaw cycles.
Uniprot
P10747
Format
Aqueous buffer solution.
Purification
Protein A affinity chromatography from HEK293 supernatants.