Anti-CD20 is a recombinant monoclonal antibody which features the constant region of the human IgG1 isotype and the variable region of rituximab. It binds to human CD20 extracellular region a.a 142- 184, where it acts asan agonist of the CD20 protein. Itis expressed in a 293 freestyle cell expression system and purified using protein A chromatography. MW = 150 kDa.
assay conditions: WIL2-S cells weretreated with anti-CD20 + 10% rabbitcomplement. Cell viability was determinedusing AlamarBlue Indicator.
Endotoxin level < 1 EU/ ug.
Formulation
10 mM PBS pH 7.4, 138 mM NaCl, and 2.8 mM KCl.
Isotype
IgG1kappa
Molecular Weight
150 kDa
Species
Human
Species Reactivity
Human
Synonyms
rituximab
Tag
none
Note
Avoid freeze/thaw cycles.
Uniprot
P11836
Format
Aqueous buffer solution
Purification
Protein A affinitychromatography from HEK293supernatants.