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Home / Primary Antibodies / Polyclonal Antibodies

Anti-c-Kit/Kit Antibody Picoband®

SKU : BHA21002349

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Mfr.No.	A01335-1, A01335-1-10ug, A01335-1-Biotin, A01335-1-Cy3, A01335-1-Dylight488, A01335-1-Dylight550, A01335-1-Dylight594, A01335-1-FITC, A01335-1-HRP, A01335-1-APC, A01335-1-PE, A01335-1-iFluor647, A01335-1-carrier-free
Alternative Names	KIT proto-oncogene, receptor tyrosine kinase
Background	SCFR (Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-c-Kit/Kit Antibody Picoband® catalog # A01335-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration a
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of mouse c-Kit/Kit, which shares 88.6% and 94.3% amino acid (aa) sequence identity with human and rat c-Kit/Kit, respectively.
Isotype	Rabbit IgG
Molecular Weight	145 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT; SCFR
Tissue Specificity	Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P05532
Gene Id	3815
Gene Name	Kit

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Weste blot, 0.25-0.5ug/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat<br>

Reference	1. Barker, P. E., Besmer, P., Ruddle, F. H. Human c-kit oncogene on human chromosome 4. (Abstract) Am. J. Hum. Genet. 37: A143, 1985.<br /><br />2. Chen, L. L., Sabripour, M., Wu, E. F., Prieto, V. G., Fuller, G. N., Frazier, M. L. A mutation-created novel intra-exonic pre-mRNA splice site causes constitutive activation of KIT in human gastrointestinal stromal tumors. Oncogene 24: 4271-4280, 2005.<br /><br />3. Andre C, Hampe A, Lachaume P, Martin E, Wang XP, Manus V, Hu WX, Galibert F (January 1997). "Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes". Genomics 39(2): 21626.<br /><br />4. Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U, Ullrich A (November 1987). "Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand". EMBO J. 6 (11): 334151.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002349-1	A01335-1	-	100 ug	$370		Add to Cart
BHA21002349-2	A01335-1-10ug	Sample	10 ug	$99		Add to Cart
BHA21002349-3	A01335-1-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002349-4	A01335-1-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002349-5	A01335-1-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002349-6	A01335-1-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002349-7	A01335-1-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002349-8	A01335-1-FITC	FITC	100 ug	$570		Add to Cart
BHA21002349-9	A01335-1-HRP	HRP	100 ug	$570		Add to Cart
BHA21002349-10	A01335-1-APC	APC	100 ug	$670		Add to Cart
BHA21002349-11	A01335-1-PE	PE	100 ug	$670		Add to Cart
BHA21002349-12	A01335-1-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002349-13	A01335-1-carrier-free	carrier-free	100 ug	$370		Add to Cart

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