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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband®

SKU : BHA21003288

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Mfr.No.	A04085-3, A04085-3-10ug, A04085-3-Biotin, A04085-3-Cy3, A04085-3-Dylight488, A04085-3-Dylight550, A04085-3-Dylight594, A04085-3-FITC, A04085-3-HRP, A04085-3-APC, A04085-3-PE, A04085-3-iFluor647, A04085-3-carrier-free
Alternative Names	cholinergic receptor muscarinic 1
Background	Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband® catalog # A04085-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi tu
Host	Rabbit
Immunogen Region	E.coli-derived human Aspartate Aminotransferase/GOT1 recombinant protein (Position: S5-Q413).
Isotype	Rabbit IgG
Molecular Weight	41 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Muscarinic acetylcholine receptor M1; CHRM1
Tissue Specificity	Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Uniprot	P17174
Gene Id	2805
Gene Name	GOT1
Subcellular Location	Cell membrane. Multi-pass membrane protein. Postsynaptic cell membrane. Multi-pass membrane protein.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Aitken, D. A., Ferguson-Smith, M. A. Gene dosage evidence for the regional assignment of the GOT-S structural gene locus to 10q24-10q25. Cytogenet. Cell Genet. 22: 468-471, 1978. <br /><br />2. Creagan, R., Tischfield, J., McMorris, F. A., Chen, S.-H., Hirschi, M., Chen, T.-T., Ricciuti, F., Ruddle, F. H. Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybrids. Cytogenet. Cell Genet. 12: 187-198, 1973. <br /><br />3. Doonan, S., Barra, D., Bossa, F. Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int. J. Biochem. 16: 1193-1199, 1984.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003288-1	A04085-3	-	100 ug	$370		Add to Cart
BHA21003288-2	A04085-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21003288-3	A04085-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003288-4	A04085-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003288-5	A04085-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003288-6	A04085-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003288-7	A04085-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003288-8	A04085-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21003288-9	A04085-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21003288-10	A04085-3-APC	APC	100 ug	$670		Add to Cart
BHA21003288-11	A04085-3-PE	PE	100 ug	$670		Add to Cart
BHA21003288-12	A04085-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003288-13	A04085-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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