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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Aquaporin 2/AQP2 Antibody Picoband®

SKU : BHA21007022

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Mfr.No.	PA1742, PA1742-10ug, PA1742-Biotin, PA1742-Cy3, PA1742-Dylight488, PA1742-Dylight550, PA1742-Dylight594, PA1742-FITC, PA1742-HRP, PA1742-APC, PA1742-PE, PA1742-iFluor647, PA1742-carrier-free
Alternative Names	Aquaporin-2
Background	AQP2 (Aquaporin 2) also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Aquaporin 2/AQP2 Antibody catalog # PA1742. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2, identical to the related rat and mouse sequences.
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Rat, Mouse, Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Aquaporin-2;AQP-2;ADH water channel;Aquaporin-CD;AQP-CD;Collecting duct water channel protein;WCH-CD;Water channel protein for renal collecting duct;AQP2;
Tissue Specificity	Expressed in renal collecting tubules.
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Uniprot	P41181
Gene Name	AQP2
Sequence	Belongs to the MIP/aquaporin (TC 1.A.8) family.
Subcellular Location	Apical cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi- pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Rat, Human, Mouse<br>Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Rat, Human, Mouse, By Heat<br>Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br>

Reference	1. Bouley, R., Breton, S., Sun, T., McLaughlin, M., Nsumu, N. N., Lin, H. Y., Ausiello, D. A., Brown, D. Nitric oxide and atrial natriuretic factor stimulate cGMP-dependent membrane insertion of aquaporin 2 in renal epithelial cells. J. Clin. Invest. 106: 1115-1126, 2000.<br /><br />2. Canfield, M. C., Tamarappoo, B. K., Moses, A. M., Verkman, A. S., Holtzman, E. J. Identification and characterization of aquaporin-2 water channel mutations causing nephrogenic diabetes insipidus with partial vasopressin response. Hum. Molec. Genet. 6: 1865-1871, 1997.<br /><br />3. Deen, P. M. T., Croes, H., van Aubel, R. A. M. H., Ginsel, L. A., van Os, C. H. Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing. J. Clin. Invest. 95: 2291-2296, 1995.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21007022-1	PA1742	-	100 ug	$370		Add to Cart
BHA21007022-2	PA1742-10ug	Sample	10 ug	$99		Add to Cart
BHA21007022-3	PA1742-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21007022-4	PA1742-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21007022-5	PA1742-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21007022-6	PA1742-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21007022-7	PA1742-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21007022-8	PA1742-FITC	FITC	100 ug	$570		Add to Cart
BHA21007022-9	PA1742-HRP	HRP	100 ug	$570		Add to Cart
BHA21007022-10	PA1742-APC	APC	100 ug	$670		Add to Cart
BHA21007022-11	PA1742-PE	PE	100 ug	$670		Add to Cart
BHA21007022-12	PA1742-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21007022-13	PA1742-carrier-free	carrier-free	100 ug	$370		Add to Cart

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